全玻璃体液清扫Vitreodynamic分析
Summary
The goal of this protocol is to show an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes.
Abstract
The authors propose an effective technique to isolate whole, intact vitreous core and cortex from post mortem enucleated porcine eyes. While previous studies have shown the results of such dissections, the detailed steps have not been described, precluding researchers outside the field from replicating their methods. Other studies harvest vitreous either through aspiration, which does not maintain the vitreous structure anatomy, or through partial dissection, which only isolates the vitreous core. The proposed method isolates the whole vitreous body, with the vitreous core and cortex intact, while maintaining vitreous anatomy and structural integrity. In this method, a full thickness scleral flap in an enucleated porcine eye is first created and through this, the choroid tissue can be separated from the sclera. The scleral flap is then expanded and the choroid is completely separated from the sclera. Finally the choroid-retina tissue is peeled off the vitreous to leave an isolated intact vitreous body. The proposed vitreous dissection technique can be used to study physical properties of the vitreous humor. In particular, this method has significance for experimental studies involving drug delivery, vitreo-retinal oxygen transport, and intraocular convection.
Introduction
该方法的目的是对细节的技术以分离一个整体，完好玻璃体，与玻璃体芯和皮层完好，从尸体眼睛，对于vitreodynamic分析的目的。作为玻璃体生理学领域已经成长，多学科的研究者，如流体力学研究人员，正在研究玻璃体1的物理和力学性能。为此，有必要对细节的技术以分离的整体，完好玻璃体，以帮助多学科研究。
塞巴格等 2等3进行优雅整个玻璃体解剖上人尸体眼睛和显示结果的说明。然而，所使用的技术中的细节和非专家没有描述将不能够独立复制的方法。其他研究也收获了使用简单的方法，如吸入或局部解剖尸体眼玻璃体，这两者不会导致一个整体，完好玻璃体。 Gisladottis 等 4和Xu 等 5探讨通透性玻璃体从尸眼收获。然而，由于玻璃体提取无法进行了说明，人们认为它们吸出玻璃体用注射器。瓦等 6更进一步通过描述分离兔眼玻璃体液与手术技术的方法。然而，这种方法会导致在刚玻璃体芯的隔离，而不是玻璃体皮质。 Skeie 等 7后举办的玻璃体到4个独特的区域和优雅描述的方法来剖析出各个部分进行分析。这种技术然而，这并不导致完好玻璃体作为一个整体。
目前的技术，以方便，目前仅在尸眼进行的生物物理实验。以前的方法，如所描述的波夫，是有限的，因为1）无完全隔离整个玻璃体，2）收获玻璃体芯和皮层匀浆，3）玻璃体解剖结构不能维持，或4）解剖技术没有充分研究人员在其他领域详述用于复制。此外，由于巩膜和脉络膜，玻璃体的可视化的不透明度在完整眼球被限制。这限制了可以在整个眼内进行的测量的精度和可行性。此外，周边玻璃体的解剖结构可混淆的玻璃体的生化和物理性能的研究。
在最近几年，玻璃体科学的身体就突飞猛进和有理由认为整个玻璃体具有不同的性质不是其各个部分。有一个在为vitreodynamics researc调查玻璃体的物理，力学，化学性能日益增长的兴趣H，其中有应用在临床医学如药物输送，玻璃体内氧8玻璃体切割术。药理vitreodynamics，它使用药理物质来操纵玻璃体，可用于改善玻璃体成果9。生物力学特性用于模拟玻璃体流体流，其可以被用来改善玻璃体内给药技术10-12。玻璃体的各部分的物理性能是至关重要的理解玻璃体视网膜氧迁移13。所提出的玻璃体切除术可用于研究完整玻璃体的各种属性。它使台式实验与更好的可视化进行整体上的，完整的玻璃体。
总之，目前的方法对于玻璃体的研究要么没有充分描述的，或导致不完全的隔离玻璃体芯和皮层。因此，有必要以执行电子在一个透明的眼睛模型xperiments同时保持存在于尸体眼睛玻璃体的解剖结构。
Protocol
Representative Results
Discussion
有迹象表明，必须在玻璃体切除认真执行两个关键步骤。步骤3中，创建完整厚度巩膜瓣，关键的是要在整个清扫。应注意建立的全部厚度巩膜瓣时不切入脉络膜。另一个关键的步骤是解剖远离脉络膜巩膜。这一步必须谨慎进行，以防止在创建从中可以玻璃洒出脉络膜多个孔。有一种方法来修改协议，仍然完好无损解剖全玻璃体。步骤6可以被延迟，直到步骤8的脉络膜可以在末端被除去后玻璃体组?…
Declarações
The authors have nothing to disclose.
Acknowledgements
The authors acknowledge the following funding sources, Whittier Foundation, Harrington Foundation, National Institutes of Health and Research to Prevent Blindness.
Materials
| 0.3 forceps | Storz Opthalmics | E1793 | |
| Westcott Tenotomy Scissors Curved Right | Storz Opthalmics | E3320 R | |
| Scalpel Handle No. 3 | VWR | 25607-947 | |
| Scalpel Blade, #11, for #3 Handle | VWR | 470174-844 |
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