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JoVE Journal Biology
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
 

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

Article DOI: 10.3791/1553-v • 12:10 min • December 8th, 2009
December 8th, 2009 •
1, 1, 1, 1, 1, 1
1Research and Development, Stemgent

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We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific markers.

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Induced Pluripotent Stem Cells Reprogramming Human Fibroblasts Stemgent Human TF Lentivirus Set Retrovirus-mediated Delivery Oct4 Sox2 C-Myc Klf4 Pluripotent State Mouse Fibroblasts Human Somatic Cells IPS Cells Transcription Factors Retroviral Vectors James Thomson Nanog Lin28 ES Cells Morphology Proliferation Differentiation Patient-specific IPS Cells Personalized Regenerative Medicine Therapies
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