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In vivo-like Organotypic Murine Retinal Wholemount Culture
 

In vivo-like Organotypic Murine Retinal Wholemount Culture

Article DOI: 10.3791/1634-v • 25:27 min • January 11th, 2010
January 11th, 2010 •
1, 1
1Institute for Anatomy, Department of Neuroanatomy, University of Duisburg-Essen

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This video article demonstrates the establishment of organotypic retinal wholemount cultures and a cytospin procedure for analysis of exogenously induced effects. Organotypic retinal wholemount cultures mimic the in vivo situation and significantly facilitate the accessibility of murine retinas for experimental manipulations while circumventing the disadvantages of classical murine animal models.

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In Vivo-like Organotypic Murine Retinal Wholemount Culture Targeted Gene Ablations Animal Models Retinal Gene Function Transgenic Mouse Models Conditional Knockout Mouse Models Primary Cell Culture Recombinant Factors Gene Overexpression SiRNA-mediated Gene Knockdown Controlled Environment Dissociated Cell Culture Endogenous Signals Exogenously Triggered Effects Pharmacological Manipulation Organotypic Retinal Wholemount Cultures Physiological In Vivo Situation Neuronal Interactions Connections Preserved Dissection Peculiarities Embryonic Eyes Postnatal Eyes Adult Murine Eyes Neuronal Apoptosis Retinal Cell Proliferation
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