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Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
 

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

Article DOI: 10.3791/1685-v • 12:30 min • February 4th, 2010
February 4th, 2010 •
1,2,3, 1,2, 1,2,4
1Department of Otology and Laryngology, Harvard Medical School, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders, Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard

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This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.

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Primary Culture Plasmid Electroporation Murine Organ Of Corti Sensory Epithelium Audition Cochlea Inner Ear Hair Cells Mechanosensory Cells Inner Hair Cells Outer Hair Cells Basilar Membrane Sensorineural Hearing Loss Auditory Research Organ Explant Gene Expression Stereocilia Motility Cell And Molecular Biology Hair Cell Regeneration Isolation And Culture Method Neonatal Mice Temporal Bone Isolation
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