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Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
 

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Article DOI: 10.3791/57649-v • 06:38 min • May 3rd, 2018
May 3rd, 2018 •
*1, *1, 1, 1
1Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz
* These authors contributed equally

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We describe a novel method for the delivery of DNA plasmid into the urothelial cells of mouse bladder in vivo through urethra catheterization and electroporation. It offers a fast and convenient way for generating autochthonous mouse models of bladder diseases.

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Keywords: Plasmid DNA Delivery Bladder Urothelium Electroporation Gene Overexpression Genome Editing Bladder Cancer Gene Therapy Anesthesia Catheter PBS Washing DNA Plasmid Trypan Blue Vertical Incision
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