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Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
 

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

Article DOI: 10.3791/58626-v • 10:19 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 3, 1, 3
1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, 2Department of Biomedical Engineering, Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science, Washington University School of Medicine

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Resumo
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This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.

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Keywords: Extracellular Flux Analysis Energy Metabolism Retinal Tissue Glycolysis Oxidative Phosphorylation Mitochondrial Stress Mouse Retina Tissue Isolation
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