Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Assessment of Cellular Bioenergetics in Mouse…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
 

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Article DOI: 10.3791/63045-v • 10:17 min • September 24th, 2021
September 24th, 2021 •
1, 2, 2,3,4, 1,4,5
1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor

Capítulos

Resumo
Automatic Translation

The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.

Tags

Cellular Bioenergetics Mouse Hematopoietic Stem Cells Glycolysis Oxidative Phosphorylation Extracellular Flux Analyzer Mitochondrial Functions High Throughput Quantification Metabolic Pathways HSC Metabolism Suspension Cells Assay Sensor Cartridge Utility Plate Assembly Calibrant
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter