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CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications
 

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Article DOI: 10.3791/64064-v • 08:32 min • August 9th, 2022
August 9th, 2022 •
*1,2, *1,3, 1,2, 1,2, 1,2, 1
1Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru, Christian Medical College campus, 2Manipal Academy of Higher Education, 3Thiruvalluvar University
* These authors contributed equally

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The present protocol describes an optimized hematopoietic stem and progenitor cell (HSPC) culture procedure for the robust engraftment of gene-edited cells in vivo.

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Keywords: CRISPR/Cas9 Gene Editing Hematopoietic Stem And Progenitor Cells Gene Therapy Ex Vivo Culture Stemness Preservation Monogenic Diseases HIV HSPC Gene Therapy Protocol Nucleofection NSG Mice NBSGW Mice
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