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JoVE Journal Bioengineering
Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points
 

Patterned Photostimulation with Digital Micromirror Devices to Investigate Dendritic Integration Across Branch Points

Article DOI: 10.3791/2003-v • 09:30 min • March 2nd, 2011
March 2nd, 2011 •
*1, *1, 1, 1
1Department of Neurology, Baltimore VA Medical Center, University of Maryland School of Medicine
* These authors contributed equally

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Resumo
Automatic Translation

Digital micromirror devices (DMD) can generate complex patterns in time and space with which to control neuronal excitability. Issues relevant to the design, construction, and operation of DMD systems are discussed. Such a system enabled the demonstration of non-linear integration across distal dendritic branch points.

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Patterned Photostimulation Digital Micromirror Devices Dendritic Integration Neuronal Excitability Light-sensitive Effectors Channel-rhodopsin Caged Neurotransmitters Experimental Neuroscience Confocal Microscopy 2-photon Microscopy Diffraction Limited Spot Scanning Photostimulation Imaging Spatial Pattern Target Projection Hardware Components Upright Microscopes UV Photolysis Visible Light System Optogenetic Experiments
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