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JoVE Journal Bioengineering
Preparation of Complaint Matrices for Quantifying Cellular Contraction
 
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Preparation of Complaint Matrices for Quantifying Cellular Contraction

Article DOI: 10.3791/2173-v • 11:39 min • December 14th, 2010
December 14th, 2010 •
1, 1, 2, 3, 1,2
1Institute for Biophysical Dynamics, University of Chicago, 2Physics Department - James Franck Institute, University of Chicago, 3Interdisciplinary Scientist Training Program, University of Chicago

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In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.

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Complaint Matrices Quantifying Cellular Contraction Cellular Adhesion Extracellular Matrix (ECM) Focal Adhesions F-actin Cytoskeleton Mechanical Forces Cell Migration ECM Remodeling Cell Culture Substrates Traction Force Microscopy (TFM) Substrate Deformations Cytoskeletal Organization Fluorescence Microscopy
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