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Immunology and Infection

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JoVE Journal Immunology and Infection
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
 

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Article DOI: 10.3791/2308-v • 09:10 min • October 18th, 2010
October 18th, 2010 •
1, 2
1Center for Meat Safety and Quality, Department of Animal Sciences, Colorado State University, 2Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition, Iowa State University

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This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).

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Adhesive-tape-based Sampling Fluorescence In Situ Hybridization Rapid Detection Salmonella Fresh Produce Culture-independent Detection Cell-charged Tapes Solid-phase Enrichment Liquid Surface Miniculture DNA Probe Cocktail Hybridization Fluorescence Microscopy
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