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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
 

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Article DOI: 10.3791/62067-v • 05:23 min • December 23rd, 2020
December 23rd, 2020 •
1, 2, 1, 1,2, 1,2, 3,4, 3,4, 1
1Malopolska Centre of Biotechnology, Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

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Here, we describe a simple method that combines RNA fluorescence in situ hybridization (RNA-FISH) with immunofluorescence to visualize severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. This protocol may increase understanding of the molecular characteristics of SARS-CoV-2 RNA-host interactions at a single-cell level.

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Keywords: SARS-CoV-2 Immuno RNA-Fluorescence In Situ Hybridization Viral Pathogenesis Viral Replication Single-cell Analysis Diagnostics Cell Lines Human Airway Epithelium Fixation Permeabilization Amplification Buffer Hairpin Probes Snap-cooling Mounting Medium Antifade Preamplification
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