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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus
 

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Article DOI: 10.3791/3087-v • 16:27 min • September 14th, 2011
September 14th, 2011 •
1, 1, 1,2
1Brigham and Women's Hospital / Harvard Medical School, Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering, Cornell University

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We present two independent, microscope-based tools to measure the induced nuclear and cytoskeletal deformations in single, living adherent cells in response to global or localized strain application. These techniques are used to determine nuclear stiffness (i.e., deformability) and to probe intracellular force transmission between the nucleus and the cytoskeleton.

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Biophysical Assays Mechanical Properties Interphase Cell Nucleus Substrate Strain Application Microneedle Manipulation Eukaryotic Cells Nucleus Stiffness Cytoskeleton Biomechanical Behavior Physiological Conditions Pathological Conditions Migrating Neutrophils Invading Cancer Cells Extravasation Narrow Spaces Mechanically Active Tissue Muscle Structural Support Repetitive Mechanical Stress Cellular Architecture Nuclear Envelope Proteins Lamins Nesprins Nucleo-cytoskeletal Coupling Human Diseases
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