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Journal / Neuroscience / Live Imaging of Dorsal Root Axons after Rhizotomy
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JoVE Journal Neuroscience
Live Imaging of Dorsal Root Axons after Rhizotomy
 
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Live Imaging of Dorsal Root Axons after Rhizotomy

Article DOI: 10.3791/3126-v • 06:40 min • September 1st, 2011
September 1st, 2011 •
1, 2,3, 4
1Temple University, Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service, Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy, Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine

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An in vivo imaging protocol to monitor primary sensory axons following dorsal root crush is described. The procedures utilize wide-field fluorescence microscopy and thy1-YFP transgenic mice, and permit repeated imaging of axon regeneration over 4 cm in the PNS and axon interactions with the interface of the CNS.

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Live Imaging Dorsal Root Axons Rhizotomy Spinal Root Injuries Chronic Pain Sensory Loss Regeneration Dorsal Root Entry Zone (DREZ) Molecular Events Cellular Events Nerve Injury Postmortem Analyses Axon Regeneration Real-time Events Living Animal Neurons Serially In Vivo Optics Mouse Transgenics Thy1-GFP Transgenic Mice Fluorescent Colors In Vivo Imaging Muscle Brain Physiological Mechanisms Static Analyses Spinal Cord Imaging
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