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Bioengineering

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JoVE Journal Bioengineering
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
 

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

Article DOI: 10.3791/3349-v • 12:43 min • January 17th, 2012
January 17th, 2012 •
1, 2, 2, 3, 1, 1, 2, 2, 1, 4, 2, 1
1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center

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We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.

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Parallel-plate Flow Chamber Continuous Flow Circuit Endothelial Progenitor Cells Laminar Flow Shear Stress Cell Adhesion Cell Morphology Human Umbilical Cord Blood-derived Endothelial Progenitor Cells Porcine EPCs Smooth Muscle Cells Fibroblasts Microfluidic Chambers Sterilization Cell Recovery DNA Extraction RNA Extraction Immunohistochemistry Scanning Electron Microscopy Shear Stress Adjustment Perfusate Flow Rate Fluid Viscosity Channel Height And Width
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