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Journal / Medicine / MALDI Imaging Mass Spectrometry of Neuropeptides…
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MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease
 

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease

Article DOI: 10.3791/3445-v • 16:57 min • February 14th, 2012
February 14th, 2012 •
1,2, 1, 1
1Department of Pharmaceutical Biosciences, Uppsala University, 2Department of Chemical and Biological Engineering, Chalmers University of Technology

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Resumo
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Dopamine replacement pharmacotherapy using L-DOPA is the most commonly used symptomatic treatment of Parkinson’s disease, but is accompanied by side effects including involuntary abnormal movements, termed dyskinesia 1. Here, a protocol for MALDI imaging mass spectrometry is presented that detects changes in rat brain neuropeptide levels related to dyskinesia.

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MALDI Imaging Mass Spectrometry Neuropeptides Parkinson's Disease IMS Molecular Species Tissue Samples MALDI Matrix Intact Peptides Proteins Mass Analyzer MALDI TOF/TOF Mass Spectrometer Rat Brain Tissue Section Imaging Techniques Molecules Of Interest Unsupervised Analysis Molecular Specificity Sensitivity Region-specific Distribution Profiles Animal Model Parkinson's Disease Neurodegenerative Disease Dopamine Replacement L-DOPA-induced Dyskinesias (LID) Opioid Precursor Prodynorphin MRNA Dynorphin Peptides Neurotransmission
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