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JoVE Journal Neuroscience
Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
 

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Article DOI: 10.3791/4008-v • 06:47 min • April 19th, 2012
April 19th, 2012 •
1, 1
1Developmental Neurobiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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Resumo
Automatic Translation

We describe a simple and low cost technique for introducing high concentration of fluorescent and calcium-sensitive dyes into neurons or any neuronal tract using a polyethylene suction pipette.

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Retrograde Labeling Nerves Tracts Spinal Roots Fluorescent Dyes Calcium-sensitive Dyes Voltage-sensitive Dyes Solid Crystals Local Pressure Injection Glass Pipettes Dye Diffusion Polyethylene Suction Pipette
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