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Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
 

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

Article DOI: 10.3791/4084-v • 14:48 min • July 6th, 2012
July 6th, 2012 •
1, 1, 1, 1,2
1Chemistry Research and Development, Luminex Corporation, 2Global Marketing, Luminex Corporation

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An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.

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ELISA Luminex XMAP Assay Antibody Screening Multiplex Capture Antibody Analytes Biological Samples Life Science Research Clinical Diagnostics Limitations 96-well Microplate Sample Volume Antigen Non-specific Binding Background Enzyme-mediated Amplification Sensitivity Technology Higher Throughput Flexibility Reduced Cost Workflow Luminex XMAP Technology Microsphere Array Platform Monoplex Assay Protein Applications Nucleic Acid Applications
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