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JoVE Journal Bioengineering
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
 

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Article DOI: 10.3791/4176-v • 15:29 min • November 16th, 2012
November 16th, 2012 •
1, 2, 3, 1, 4
1UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1, Université de Lyon, 2Département Biosciences, INSA de Lyon, Université de Lyon, 3INSERM U758, Ecole Normale Supérieure de Lyon, Université de Lyon, 4Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon, Université de Lyon

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Resumo
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The design of a synthetic operon encoding both the secretory apparatus and the structural monomers of curli fibers is described. Overproduction of these amyloids and adherent polymers allows a measurable gain of adherence of the E. coli chassis1. Easy ways to visualize and quantify adherence are explained.

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Engineering Adherent Bacteria Synthetic Curli Operon E. Coli Promoter Visualization Quantification Bacterial Adherence Synthetic Biology BBa K540000 Biobrick Transcriptional Signals Translational Signals Curli Regulation Metal-regulated Promoter Qualitative Assessment Quantitative Assessment Biofilm Formation
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