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A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field
 

A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field

Article DOI: 10.3791/4193-v • 11:00 min • October 13th, 2012
October 13th, 2012 •
1, 2, 3
1Institute for Biomaterials and Biomedical Engineering, University of Toronto, 2Lyndhurst Centre, Toronto Rehabilitation Institute, 3Department of Surgery, University of Toronto

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Resumo
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In this protocol we demonstrate how to construct custom chambers that permit the application of a direct current electric field to enable time-lapse imaging of adult brain derived neural precursor cell translocation during galvanotaxis.

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Galvanotaxis Assay Neural Precursor Cell Migration Externally Applied Direct Current Electric Field Neural Stem Cells Neuroregenerative Strategies Mobilization Of NPCs Lesion Site Endogenous Precursors Periventricular Region Mechanisms Of NPC Migration Direct Current Electric Fields (dcEFs) Galvanotaxis Phenomenon Physiological Electric Fields Cell Migration Normal Development Wound Repair
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