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Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
 

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Article DOI: 10.3791/4449-v • 10:55 min • November 5th, 2012
November 5th, 2012 •
*1, *1, 1, 1, 1
1Department of Biology, University of North Carolina at Charlotte
* These authors contributed equally

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Xenopus egg extract is a useful model system to investigate the DNA damage checkpoint. This protocol is for the preparation of Xenopus egg extracts and DNA damage checkpoint inducing reagents. These techniques are adaptable to a variety of DNA damaging approaches in the study of the DNA damage checkpoint signaling.

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DNA Damage Checkpoint Xenopus Egg Extracts Endogenous Insults Environmental Insults DNA Damage Checkpoint Signaling Sensors Transducers Effectors ATR (ATM And Rad3-related) Kinases Chk1 (Checkpoint Kinase 1) Cell Cycle Arrest Transcription Activation DNA Damage Repair Apoptosis Senescence Genomic Instability Genomic Integrity Human Diseases Cancer Development Xenopus Laevis Egg Extracts Cell-free Extract Model System
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