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JoVE Journal Bioengineering
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
 

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Article DOI: 10.3791/50176-v • 08:51 min • March 1st, 2013
March 1st, 2013 •
1,2, 1,2, 1,2,3,4
1Biomedical Engineering Department, Johns Hopkins University School of Medicine, 2Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, 3Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Institute for Nanobiotechnology, Johns Hopkins University School of Medicine

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Resumo
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A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.

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Keywords: Polymeric Gene Delivery Nanoparticle Tracking Analysis High-throughput Flow Cytometry Non-viral Gene Delivery Cationic Polymers Polylysine Polyphosphoesters Poly(amidoamines) Polyethylenimine (PEI) Poly(beta-amino Ester)s (PBAEs) Human Retinal Endothelial Cells (HRECs) Transfection Efficiency 96-well Plate Assay
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