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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifizierung neuer Gene, die mit Alginat-Produktion verbunden in
 
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Identifizierung neuer Gene, die mit Alginat-Produktion verbunden in

Article DOI: 10.3791/51346-v • 08:57 min • March 10th, 2014
March 10th, 2014 •
1, 1, 1
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine, Marshall University

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Hier beschreiben wir ein Protokoll, mit dem Mini-himar1 mariner Transposon-vermittelte Mutagenese zur Erzeugung einer hohen Dichte Insertionsmutante Bibliothek-Bildschirm, zu isolieren und zu identifizieren Roman Alginat-Regulatoren in der prototypischen Pseudomonas aeruginosa Stamm PAO1.

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Immunologie , Alginat mucoidy Mutagenese Mini- PFAC
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