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Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells
 

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Article DOI: 10.3791/51897-v • 11:20 min • October 16th, 2014
October 16th, 2014 •
1, 1, 1, 1, 1, 1, 1, 1
1Department of Biochemistry, University of Leicester

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The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.

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Keywords: Recombinant Protein Expression Structural Biology HEK 293F Suspension Cells Eukaryotic Expression Protein Purification Post-translational Modifications Protein Complexes Transient Transfection Mammalian Cell Lines
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