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JoVE Journal Medicine
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
 

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Article DOI: 10.3791/52350-v • 11:27 min • February 7th, 2015
February 7th, 2015 •
1, 1, 1, 1, 2, 1
1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest School of Medicine, 2Seahorse Biosciences

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Resumo
Automatic Translation

Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

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Keywords: Mitochondria Skeletal Muscle Percutaneous Needle Biopsy Respirometry Extracellular Flux Analysis Electron Transport Chain COX IV GAPDH
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