JoVE Journal
Developmental Biology
Developmental Biology
This Conteúdo is Open Access.
Capítulos
Resumo
With the intent of characterizing changes in miRNAs on differentiated human neural stem cells (hNSCs) we describe hNSC differentiation on a three dimensional system,the evaluation of changes in microRNA expression by miRNA PCR array, and computational analysis for miRNA target prediction and its validation by dual luciferase assay.