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Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
 

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Article DOI: 10.3791/52605-v • 09:43 min • May 25th, 2015
May 25th, 2015 •
1,2, 1, 2, 1
1Department of Neuroscience, Farber Institute for Neurosciences, Sidney Kimmel Medical College at Thomas Jefferson University, 2Department of Biology, Arcadia University

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Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

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Protocol Functional Assessment Morphological Assessment Diaphragm Innervation Phrenic Motor Neurons Electrophysiological Level Morphological Level Compound Muscle Action Potential (CMAP) Phrenic Nerve Stimulation Functional Diaphragm Innervation Cervical Spinal Cord Anesthetized Rats Anesthetized Mice Phenotypes Motor Axons Myofibers Diaphragm NMJ (neuromuscular Junction) Disease-relevant Morphological Changes Partial Denervation Complete Denervation Regenerative Sprouting Reinnervation Whole-mount Immunohistochemistry (IHC) Diaphragm Analysis Rodent Models CNS Disease PNS Disease
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