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Isolering af human lymfeendotelceller af Multi-parameter Fluorescens-aktiveret cellesortering
 
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Isolering af human lymfeendotelceller af Multi-parameter Fluorescens-aktiveret cellesortering

Article DOI: 10.3791/52691-v • 07:36 min • May 1st, 2015
May 1st, 2015 •
1,2, 1,3, 1, 1,2,3, 1,2, 1,2,3
1Murdoch Childrens Research Institute, The Royal Children’s Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton

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Målet med denne protokol er at isolere lymfeendotelceller foring menneskelige lymfatisk misdannelse cyste-lignende fartøjer og Forhud anvender fluorescens-cellesortering (FACS). Efterfølgende celledyrkning og udvidelse af disse celler tillader et nyt niveau af eksperimentel raffinement for genetiske, proteomiske, funktionelle og celle differentiering undersøgelser.

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Medicin , flowcytometrisk sortering, celleoverflademarkører
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