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Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta
 

Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

Article DOI: 10.3791/52734-v • 08:08 min • June 10th, 2015
June 10th, 2015 •
1,2, 1, 4, 1,2,3, 1
1Departments of Physiology, Medical College of Wisconsin, 2Human and Molecular Genetics Center, Medical College of Wisconsin, 3Cardiovascular Center, Medical College of Wisconsin, 4Blood Research Institute of Wisconsin

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Vascular cell functiondepends on activity of intracellular messengers. Described here is an ex vivo two photon imaging method that allows the measurement of intracellular calcium and nitric oxide levels in response to physiological and pharmacological stimuli in individual endothelial and smooth muscle cells of an isolated aorta.

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Two-photon Imaging Intracellular Calcium Handling Nitric Oxide Production Endothelial Cells Smooth Muscle Cells Isolated Rat Aorta Vascular Tissues Physiological Processes Calcium-labeling Dyes NO-labeling Dyes Two Photon Microscopy Vasoconstrictors Vasodilators Blood Vessels Calcium Transients NO Production Two Photon Microscope Physiological Stimuli Pharmacological Stimuli Endothelial Cells Smooth Muscle Cells
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