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Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
 

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Article DOI: 10.3791/54014-v • 09:39 min • May 19th, 2016
May 19th, 2016 •
*1, *1, 2, 1
1Department of Cellular and Molecular Physiology, College of Medicine, Penn State University, 2Lane Department of Computer Science and Electrical Engineering, West Virginia University
* These authors contributed equally

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Primary human umbilical vein endothelial cells (HUVECs) were grown to confluence within a microfluidic network device. The endothelial cell junction and F-actin distributions were illustrated and the changes in intracellular calcium concentration and nitric oxide production in response to adenosine triphosphate (ATP) were quantified in real-time at individual cell levels.

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Development Characterization In Vitro Microvessel Network Quantitative Measurements Endothelial [Ca2+]i Nitric Oxide Production Microfluidic Device Shear Flow Agonist Induced Changes Calcium Versatile Design Microchannels Vasculature Cell Culture Environment Photolithography Master Mold Soft Lithography PDMS Microchannel Devices Fibronectin PBS
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