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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
 

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Article DOI: 10.3791/52836-v • 11:15 min • May 21st, 2015
May 21st, 2015 •
1, 1, 1, 1
1Department of Oral Microbiology and Immunology, School of Dentistry and Dental Research Institute, Seoul National University

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Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.

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Keywords: In Situ Detection Bacteria Paraffin-embedded Tissues Digoxin-labeled DNA Probe 16S RRNA Periodontitis Gingival Biopsies Electron Microscopy Immunohistochemistry Immunofluorescence Fluorescent In Situ Hybridization (FISH) Paraffin-embedded Tissue Species-specific Universal Bacterial Forms Epithelia Connective Tissue Inflammatory Infiltrates Blood Vessels Periodontitis Cancer Inflammatory Immune Diseases
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