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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
 

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Article DOI: 10.3791/52893-v • 13:06 min • September 24th, 2015
September 24th, 2015 •
1, 1, 1
1Department of Oncology, Faculty of Dentistry and Medicine, University of Alberta

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Electron spectroscopic imaging can image and distinguish nucleic acid from protein at nanometer resolution. It can be combined with the miniSOG system, which is able to specifically label tagged proteins in transmission electron microscopy samples. We illustrate the use of these technologies using double-strand break repair foci as an example.

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Keywords: MiniSOG DNA Repair Proteins Electron Spectroscopic Imaging (ESI) Transmission Electron Microscopy Protein Localization DNA Double-strand Break Repair Structural Information
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