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Immunology and Infection

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JoVE Journal Immunology and Infection
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
 

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Article DOI: 10.3791/53077-v • 05:12 min • August 13th, 2015
August 13th, 2015 •
1, 1,2, 1, 1
1Department of Cardiology and Angiology I, Heart Center, University of Freiburg, 2Faculty of Biology, University of Freiburg

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Resumo
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This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.

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Intravital Microscopy Leukocyte-endothelial Interactions Platelet-leukocyte Interactions Mesenterial Veins Mice Protocol Inflammatory Setting LPS Challenge Inflammatory Conditions Drugs Administration Leukocyte Recruitment Fluorescent Labeling Rhodamine 6G Targeted Fluorescently Labeled Molecules
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