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JoVE Journal Bioengineering
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
 

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Article DOI: 10.3791/53300-v • 07:23 min • June 15th, 2016
June 15th, 2016 •
1, 2, 3, 4, 4, 4, 4, 4
1Division of Hematology, Davidoff Cancer Center, Rabin Medical Center, 2The Center of Nanoscience and Nanotechnology, Tel Aviv University, 3Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, 4Department of Leukemia, The University of Texas MD Anderson Cancer Center

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Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.

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Keywords: Transcription Factor MicroRNA Transcriptome Data Mining Bioinformatic Analysis Chromatin Immunoprecipitation Sequencing H3K4me3 Epigenetic Signature Median Binding Affinity ShRNA HEK293 Cells DMEM FBS Lentivirus Packaging Vectors Transfection
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