Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / A Human Glioblastoma Organotypic Slice Culture…
JoVE Journal
Medicine

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Medicine
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
 

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Article DOI: 10.3791/53557-v • 08:35 min • July 20th, 2017
July 20th, 2017 •
1, 2, 2, 2
1Department of Neurosurgery, Stanford University Hospital, Stanford University School of Medicine, 2Inova Neuroscience Institute

Capítulos

Resumo
Automatic Translation

Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.

Tags

Keywords: Glioblastoma Organotypic Slice Culture Tumor Cell Migration Anti-invasive Drugs Neurooncology Primary Cells Tissue Architecture Cellular Heterogeneity Tissue Processing Agarose Embedding Vibratome Sectioning
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter