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Journal / Neuroscience / Preparation of Acute Brain Slices Using an…
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Neuroscience

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JoVE Journal Neuroscience
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
 

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Article DOI: 10.3791/53825-v • 10:53 min • February 26th, 2018
February 26th, 2018 •
*1, *1, 1, 1, 2, 1, 1, 1
1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute
* These authors contributed equally

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This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.

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Keywords: Brain Slices NMDG Protective Recovery Method Patch Clamp Electrophysiology Neuronal Preservation Adult Mouse Brain Tissue Slicing NMDG HEPES ACSF Brain Slice Recovery Chamber HEPES ACSF Agarose Embedding Tissue Preparation
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