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Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
 

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

Article DOI: 10.3791/54001-v • 11:45 min • May 29th, 2016
May 29th, 2016 •
1, 1, 1
1Department of Biochemistry, University of Cambridge

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Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.

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Keywords: Cyanobacteria Synechocystis Synechococcus Unmarked Mutants Marked Mutants Antibiotic Resistance Negative Selectable Marker Genetic Manipulation BG11 Medium Transformation Kanamycin PCR Knockout
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