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Immunology and Infection

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JoVE Journal Immunology and Infection
Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing
 

Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

Article DOI: 10.3791/54210-v • 10:29 min • July 6th, 2016
July 6th, 2016 •
1, 1, 1
1Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne

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Investigating the interactions between bacterial pathogens and their hosts is an important area of biological research. Here, we describe the necessary techniques to measure effector translocation by Coxiella burnetii during siRNA gene silencing using BlaM substrate.

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Keywords: FRET Effector Translocation SiRNA Silencing Coxiella Burnetii Cellular Microbiology Host-pathogen Interactions Chlamydia Salmonella E. Coli Beta-lactamase ACCM2 HeLa Cells Transfection 96-well Plate
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