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Assays for the Degradation of Misfolded Proteins in Cells
 

Assays for the Degradation of Misfolded Proteins in Cells

Article DOI: 10.3791/54266-v • 10:56 min • August 28th, 2016
August 28th, 2016 •
1,2, 1, 1
1Department of Cancer Biology, University of Pennsylvania Perelman School of Medicine, 2Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania Perelman School of Medicine

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This report describes protocols for measuring degradation rates of misfolded proteins by either western blot or fluorescence-based assays. The methods can be applied to analysis of other misfolded proteins and for high throughput screening.

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Keywords: Misfolded Proteins Protein Degradation Protein Quality Control Neurodegenerative Diseases Atxn1 82Q Nuclear Luciferase Cell Fractionation Cycloheximide Proteasome Inhibitor MG132 Cell Lysis Centrifugation
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