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Journal / Developmental Biology / Ploiditet Manipulering av zebrafiskembryon med…
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JoVE Journal Developmental Biology
Ploiditet Manipulering av zebrafiskembryon med Heat Shock 2 Behandling
 
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Ploiditet Manipulering av zebrafiskembryon med Heat Shock 2 Behandling

Article DOI: 10.3791/54492-v • 11:19 min • December 16th, 2016
December 16th, 2016 •
1, *1,2, *1,3, 1
1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine
* These authors contributed equally

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En modifierad protokoll för ploidi manipulation använder en värmechock för att inducera en cykel stall i cytokines i det tidiga embryot. Detta protokoll demonstreras i zebrafisk, men kan tillämpas på andra arter.

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Utvecklingsbiologi zebrafisk ploiditet manipulation hela genomet dubblering gynogenesis värmechock centriol dubbel homozygot diploid
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