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Adenofection
 

Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments

Article DOI: 10.3791/54557-v • 12:57 min • September 16th, 2016
September 16th, 2016 •
1,2, 3,4, 1,2, 1,2, 1,2
1Département de biologie moléculaire, biochimie médicale et pathologie, Faculté de médecine, Centre de recherche sur le cancer de l'Université Laval, 2Oncology, Centre de recherche du CHU de Québec, Université Laval, 3Laboratoire d'études moléculaires des valvulopathies (LEMV), Groupe de recherche en valvulopathies (GRV), Quebec Heart and Lung Institute/Research Center, 4Department of Surgery, Université Laval

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We describe a method for depletion-rescue experiments that preserves cellular integrity and protein homeostasis. Adenofection enables functional analyses of proteins within biological processes that rely on finely tuned actin-based dynamics, such as mitotic cell division and myogenesis, at the single-cell level.

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Keywords: Adenofection Molecular Chaperones Cellular Morphodynamics Depletion-rescue Experiments Protein Functions Cell Dynamics Mitosis Cell Differentiation Fibronectin HeLa RFP H2B Cells Virus Transduction
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