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A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
 

A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles

Article DOI: 10.3791/3910-v • 12:25 min • August 31st, 2012
August 31st, 2012 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, University of British Columbia

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Nanodiscs are small discoid particles that incorporate membrane proteins into a small patch of phospholipid bilayer. We provide a visual protocol that shows the step-by-step incorporation of the MalFGK2 transporter into a disc.

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Nanodisc Lipid Particles Reconstitution Method ABC Transporter Membrane Proteins Ligand-receptor Interactions Amphipathic Properties Apolipoprotein A1 Hydrophobic Interactions Detergent Removal Protein-to-lipid Ratio
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