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JoVE Journal Biochemistry
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
 

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Article DOI: 10.3791/55008-v • 06:31 min • January 11th, 2017
January 11th, 2017 •
1, 1,2, 3
1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity, National Institute for Basic Biology, 2Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology, National Institute for Basic Biology

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We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

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Efficient Method Isolation Highly Purified RNA Seeds Quantitative Transcriptome Analysis Plant Seeds Cereal Biology Gene Profiles Spin Column-based Method Oils Proteins Carbohydrates Polyphenols Kazumi Hikino Technician Laboratory PVP Cell Lysis Buffer Vortex Incubation Arabidopsis Thaliana Plants
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