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JoVE Journal Biochemistry
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
 

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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We present an approach to purify ribosome-bound mRNA from vascular endothelial cells (ECs) directly in mouse brain, lung and heart tissues via EC-specific genetic tag of enhanced green fluorescence protein (EGFP)in ribosomes in combination with RNA purification.

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Keywords: RNA Isolation Endothelial Cells Ribosome Affinity Purification (TRAP) Transgenic Mice GFP-tagged Ribosomes Transcriptome Analysis Angiogenesis Epigenetics Transcription Regulation Genotyping RNA Sequencing RT-qPCR
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