JoVE Journal
Biochemistry
Biochemistry
This Conteúdo is Open Access.
Capítulos
Resumo
The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.