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JoVE Journal Developmental Biology
Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
 

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Article DOI: 10.3791/55204-v • 09:41 min • February 24th, 2017
February 24th, 2017 •
*1, *1, 1, 1, 1
1Institute of Physiology I, University of Bonn
* These authors contributed equally

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Resumo
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To distinguish cell division from cell cycle variations in cardiomyocytes, we present protocols using two transgenic mouse lines: Myh6-H2B-mCh transgenic mice, for the unequivocal identification of cardiomyocyte nuclei, and CAG-eGFP-anillin mice, for distinguishing cell division from cell cycle variations.

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Keywords: Cell Cycle Cardiomyocytes Nucleation Postnatal Visualization Cell Division Ploidy Multi-nucleation EGFP H2B-mCherry Cell Isolation Enzyme Digestion Cell Counting Cell Culture SiRNA Transfection
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