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JoVE Journal Developmental Biology
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
 

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Article DOI: 10.3791/55242-v • 12:11 min • May 11th, 2017
May 11th, 2017 •
1, 1,2, 3, 3, 4, 5, 3, 6, 1, 1, 3
1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

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This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.

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Keywords: Chromosome Segregation Histone Acetylation Spindle Morphology Horse Oocytes Oocyte Biology Fertility Gamete Manipulation OPU Procedures Follicle Maturation HCG Injection Jugular Vein Ultrasound Follicular Aspiration Cumulus-oocyte Complex Dulbecco's Modified PBS Heparin
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