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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
 

Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

Article DOI: 10.3791/55878-v • 09:20 min • August 10th, 2017
August 10th, 2017 •
*1, *1, 1, 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
* These authors contributed equally

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Resumo
Automatic Translation

The present method serves to identify isoform-specific inhibitors of histone deacetylases (HDAC) in HeLa cells by the UHPLC-MS analysis of multiple substrates. This is an antibody-free method developed to reflect HDAC1 and HDAC6 activity in the living cell environment, in contrast to single-isoform cell-free assays.

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Keywords: HDAC1 HDAC6 HeLa Cells UHPLC-MS HDAC Inhibitor Selectivity Drug Discovery Simultaneous Measurement Cell-based Assay Acetylation Trichostatin A MS275 Tubastatin A
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