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Developmental Biology

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JoVE Journal Developmental Biology
Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
 

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Article DOI: 10.3791/56002-v • 11:59 min • September 6th, 2017
September 6th, 2017 •
1, 1, 2, 2,3, 4
1Department of Pediatrics, Medical University Innsbruck, 2Department of Internal Medicine V (Hematology & Oncology), Medical University Innsbruck, 3Tyrolean Cancer Research Institute, 4Department of Pediatrics, Medical University Graz

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Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.

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Keywords: Hematopoietic Stem Cell Transplantation Hemoglobinopathies Chimerism Erythroid Progenitor Cells Flow Cytometry Short Tandem Repeats Graft Rejection Disease Relapse Immunomodulatory Strategies Cell Suspension Bone Marrow Gradient Medium Mononuclear Cells Cell Concentration Trypan Blue Stain Hemocytometer
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