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JoVE Journal Developmental Biology
Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
 

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Article DOI: 10.3791/52298-v • 12:06 min • January 28th, 2015
January 28th, 2015 •
1, 1, 2, 2, 1, 1
1Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health

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A method was developed to directly derive human neural stem cells from hematopoietic progenitor cells enriched from peripheral blood cells.

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Neural Stem Cells Peripheral Blood Hematopoietic Progenitor Cells Induced Pluripotent Stem Cells (iPSC) In Vitro Models Neurological Diseases Skin Fibroblasts Patient-specific IPSC Labor-intensive Resources Months Method Sendai Virus Vectors Transcriptional Factors Sox2 Oct3/4 Klf4 C-Myc Morphological Changes Human Neural Progenitor Medium Basic Fibroblast Growth Factor (bFGF) Vascular Endothelial Growth Factor (VEGF) Neural Stem Cell Markers
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